nucl ear dna content and dna ploidy analysis in breast cancer

نویسندگان

f sari aslani from the department of pathology, shiraz university of medical sciences, shiraz, i.r. iran.

b salimi

چکیده

to investigate the patterns of dna ploidy and proliferating activity in breast cancer and relate them to other prognostic factors, paraffin blocks of 53 cases of breast carcinoma were studied. cancer cells obtained by mechanical tissue disaggregation were examined for dna content, ploidy and s-phase fraction. dna assay was done using a cas interactive image analyzing system. all of the cases showed high degrees of proliferation. the rate of aneuploidy was 77% in invasive breast carcinomas. s-phase fractions were correlated with the grades of the tumors (p

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

NUCL EAR DNA CONTENT AND DNA PLOIDY ANALYSIS IN BREAST CANCER

To investigate the patterns of DNA ploidy and proliferating activity in breast cancer and relate them to other prognostic factors, paraffin blocks of 53 cases of breast carcinoma were studied. Cancer cells obtained by mechanical tissue disaggregation were examined for DNA content, ploidy and S-phase fraction. DNA assay was done using a CAS interactive image analyzing system. All of the cas...

متن کامل

DNA content measurement for DNA ploidy and cell cycle analysis.

This unit presents the most common methods of DNA content analysis. The techniques differ primarily in the mode of cell permeabilization (detergents versus prefixation with alcohol) and composition of the stain solution. Protocols are given for analysis of fixed cells, tissue samples, live cells (for subsequent sorting and culture), archival paraffin-embedded tissue, and apoptotic cells.

متن کامل

Analysis of nuclear DNA content and ploidy in higher plants.

This is the first of a series of units discussing the application of cytometry to plant material. Techniques commonly used for mammalian nuclei evaluation need considerable modification to be successful with plant material. David Galbraith and his colleagues bring together many years of knowledge in plant cytometry. Their unit provides detailed protocols on measuring DNA content, ploidy, and ce...

متن کامل

Detection of Toxoplasma gondii DNA in Malignant Breast Tissues in Breast Cancer Patients

Breast cancer is the most prevalent malignancy in women throughout the world. Similar to other cancers, a strong relationship between breast cancer and environmental factors such as infectious agents has been reported. Toxoplasma gondii is a protozoan parasite which may play a role in cancer induction. The present study aimed to investigate a possible association between a history of T. gondii ...

متن کامل

Intratumoral Variations in DNA Ploidy and S-Phase Fraction in Human Breast Cancer

To study intratumoral DNA ploidy heterogeneity and S-phase fraction (SPF) variability, we prospectively collected five different samples from 48 breast carcinomas and each sample was analysed separately by flow cytometry. Aneuploidy rate was 89.6% after analysis of four or five samples. DNA ploidy heterogeneity, i.e., different samples classified as either DNA euploid or DNA aneuploid in the sa...

متن کامل

Combined analysis of DNA ploidy index and N-myc genomic content in neuroblastoma.

The aim of the study was to assess, in a group of nonselected patients with neuroblastoma, the prognostic value of both N-myc gene amplification and DNA ploidy index, taking into account potential confounding factors such as age and stage. Of 59 patients studied, 23 were younger than 1 year at diagnosis, 31 presented with stage IV, 10 with stage III, 5 with stage II, 8 with stage I, and 4 with ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۱۴، شماره ۳، صفحات ۲۰۷-۲۰۹

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023